Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Nischarin (NISCH) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Description: tissue homogenates and other biological fluids.

Description: tissue homogenates and other biological fluids.

Description: tissue homogenates and other biological fluids.

Description: tissue homogenates and other biological fluids.

Description: A sandwich ELISA kit for detection of Nischarin from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Description: Recombinant Human Nischarin expressed in: E.coli

Description: Recombinant Mouse Nischarin expressed in: E.coli

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH)

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH)

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Human Nischarin (NISCH). This antibody is labeled with APC-Cy7.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with PE.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with APC.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with Cy3.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with HRP.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with FITC.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with Biotin.

Description: A Rabbit polyclonal antibody against Mouse Nischarin (NISCH). This antibody is labeled with APC-Cy7.

Description: Recombinant protein for human Nischarin

Description: Description of target: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.By similarity5 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"Imidazoline receptor antisera-selected (IRAS) cDNA: cloning and characterization."_x005F_x005F_x000D_Piletz J.E., Ivanov T.R., Sharp J.D., Ernsberger P., Chang C.-H., Pickard R.T., Gold G., Roth B., Zhu H., Jones J.C., Baldwin J., Reis D.J._x005F_x005F_x000D_DNA Cell Biol. 19:319-329(2000) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, VARIANTS ILE-299 AND VAL-1056.Ref.2"IRAS splice variants."_x005F_x005F_x000D_Piletz J.E., Deleersnijder W., Roth B.L., Ernsberger P., Zhu H., Ziegler D._x005F_x005F_x000D_Ann. N. Y. Acad. Sci. 1009:419-426(2003) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 3 AND 4), FUNCTION, TISSUE SPECIFICITY, VARIANT ILE-299.Ref.11"IRAS is an anti-apoptotic protein."_x005F_x005F_x000D_Dontenwill M., Piletz J.E., Chen M., Baldwin J., Pascal G., Ronde P., Dupuy L., Greney H., Takeda K., Bousquetd P._x005F_x005F_x000D_Ann. N. Y. Acad. Sci. 1009:400-412(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.Ref.12"IRAS, the human homologue of Nischarin, prolongs survival of transfected PC12 cells."_x005F_x005F_x000D_Dontenwill M., Pascal G., Piletz J.E., Chen M., Baldwin J., Ronde P., Dupuy L., Urosevic D., Greney H., Takeda K., Bousquet P._x005F_x005F_x000D_Cell Death Differ. 10:933-935(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION.Ref.13"Human Nischarin/imidazoline receptor antisera-selected protein is targeted to the endosomes by a combined action of a PX domain and a coiled-coil region."_x005F_x005F_x000D_Lim K.-P., Hong W._x005F_x005F_x000D_J. Biol. Chem. 279:54770-54782(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBUNIT, INTERACTION WITH ITGA5, MUTAGENESIS OF ARG-49 AND TYR-50, SUBCELLULAR LOCATION. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich Immunoassay;Sensitivity: < 0.054 ng/mL

Description: Description of target: This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.;Species reactivity: Human;Application: ;Assay info: Quantitative Sandwich ELISA;Sensitivity: < 0.067 ng/mL